Genetic Recombination in Streptomyces fradiae by Protoplast Fusion and Cell Regeneration
نویسندگان
چکیده
منابع مشابه
Protoplast fusion in Streptomyces: conditions for efficient genetic recombination and cell regeneration.
Protoplasts from four different species of Streptomyces regenerated cells efficiently in hypertonic soft agar medium overlaid on partially dehydrated regeneration medium. The efficiencies of regeneration were strongly dependent upon the incubation temperatures for cell growth and for protoplast regeneration. Cell growth temperatures (before protoplast formation) required for efficient protoplas...
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Protoplast fusion was investigated as a technique for genetically manipulating two lignin-degrading Streptomyces strains, Streptomyces viridosporus T7A and Streptomyces setonii 75Vi2. Four of 19 recombinants tested showed enhanced production of acid-precipitable polymeric lignin (APPL), producing 155 to 264% more APPL from corn stover lignocellulose than was produced by the wild-type S. viridos...
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Of basic importance to the study of antibiotic production by microorganisms is a chemically defined medium in which the effect of various nutrients, both organic and inorganic, can be studied with a minimum of interference from ill-defined substances. Such a medium is reported here for the production of neomycin by Streptomyces fradiae, together with studies on the influence of various nitrogen...
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The antibiotic fosfomycin was produced as a secondary metabolite in a glucose-asparagine medium containing citrate, l-methionine, and l-glutamate. The citrate requirement for antibiotic synthesis was related to its requirement for growth. In contrast, l-methionine and l-glutamate caused a marked stimulation of fosfomycin production and had no effect on growth. l-Methionine had to be added early...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1978
ISSN: 0022-1287
DOI: 10.1099/00221287-107-1-93